Elucidating the secretion proteome of human

31-Jul-2019 11:27 by 2 Comments

Elucidating the secretion proteome of human - for dating with phone

The use of secretome containing CM has several advantages compared to the use of stem cells, as CM can be manufactured, freeze-dried, packaged, and transported more easily.

Moreover, growth factor measures also differed, that is, pg/m L or ng/m L [6, 8, 10, 13, 17, 24, 27, 28, 33], pg/μg DNA [9, 36], fg/cell [25], spot density [5], and positive/negative [20] (Table 3).

Therefore, stem cell-derived conditioned medium have a promising prospect to be produced as pharmaceuticals for regenerative medicine.

To date, no clinical trial that used CM for a certain disease has been reported, except two pilot studies on the use of adipose derived mesenchymal stem cell CM for hair follicle regeneration [3] and fractional carbon dioxide resurfacing wound healing [4] in human, which showed good results.

The various conditioned media, even when they were derived from same kind of cells, were produced by different condition, that is, from different passage, number of cells, culture medium, and culture condition (Table 2).

The growth factor yields of the various types of cells can be seen in Table 3, and the cell number that is needed to produce CM for one application can be seen in Table 4.

Various studies on stem cell-derived secreted factors showed that the secreted factor alone without the stem cell itself may cause tissue repair in various conditions that involved tissue/organ damage.

The secreted factors are referred to as secretome, microvesicles, or exosome and can be found in the medium where the stem cells are cultured; thus, the medium is called conditioned medium (CM) [2].The use of CM for therapy is very appealing and may be booming in the near future, as studies on the use of CM for various diseases are accumulating [1, 3–33].Conditioned medium contains various growth factors and tissue regenerative agents, which were secreted by the stem cells.In the two cases of kidney disease, it can be concluded that CM from hu-ESC-MSC can improve the condition, and the needed growth factor level is presumably enough as CM processing includes a 25-time concentration step [29].However, for hu-UCB-USSC or m BM-MSC-CM, lack of data concerning CM processing and growth factor level of the CM [30] prevent further analysis to conclude whether the failure to improve the condition is due to the lack of certain growth factor or due to the level of growth factors that was too low to give an effect.We got 39 articles that met the inclusion criteria, and 7 were excluded due to incomplete data.